| 1. | Chain termination codon
链终止密码子 |
| 2. | Sv40 polya signal and two loxp sites was added consecutively after the termination codon taa , and then a gfp gene was inserted between the two loxp sites
在三个终止密码子taa之后，连上sv40polya 。 sv40polya之后，又连上两个loxp位点。在两个loxp位点，插入标记基因gfp 。 |
| 3. | The full length cdna ej175 was consisted of 603 bp to have an open reading frame from 57 to 515 including initiator codon and termination codon and to encode 152 amino acid polypeptide
将全长基因序列与genbank已知序列进行比对，其序列与拟南芥的pollenallergen - likeprotein568bp的编码序列有56的同源性。 |
| 4. | The tk gene contained an open reading frame of 957 bp encoding a 318 aa protein . upstream of the tk orf , three putative gc boxes are located at positions - 22 , - 166 and - 199 . a potential poly a signal begins 110 nucleotides downstream from the termination codon at position 1306
在tkorf上游- 22 、 - 166 、 - 199位存在3个gc框样序列，在终止密码子下游第110个核苷酸处的1306位存在有多聚腺苷加尾信号。 |
| 5. | In comparison with genbank data , the homologies of the nucleotide sequence and amino acid sequence were as following : hc was 98 . 4 % and 100 % ; ha was 97 . 2 % and 99 . 3 % respectively . 4 . two recombinant prokaryotic expression vector were constructed which has complete open reading occlusing initation codon , leader signal peptide sequence and termination codon
序列分析表明，所克隆获得的基因与genbank中已经登录的核苷酸和氨基酸的同源性分别为： hc98 . 4和100 ， ha97 . 2和99 . 3 ，证明本试验虫株与国外报道的同源性很高。 |
| 6. | In order to get small molecular g - protein rab3a , which serves to further investigate the interaction between rab3a and other proteins , we amplified the full coding region of rab3a cdna by polymerase chain reaction , using human placenta total cdna as template . the pcr products were recovered from gel electrophoresis and cloned into bamhi xhoi site of vector pyestrp2 . the result of sequencing indicated that rab3a insertion fragment included its initiation and termination codons in 5 - and 3 - terminal , respectively
为了获取全长的小分子g -蛋白rab3a ，以用于研究rab3a与其他蛋白相互作用关系，本实验以人胎盘总cdna为模板， pcr扩增到人rab3a cdna全编码区。产物回收后克隆于质粒pyestrp2的bamhi xhoi位点，测序结果表明，本实验获得的rab3a cdna包含了起始和终止密码子。 |
| 7. | The gene fragment we have got is 1311bp long , which contains a length of 543bp from 5 ' - end to the coding region , 588bp of open reading frame including " the coding region of 585bp and a termination codon as well as the 3 ' - end sequence of 180bp rich in at ( accounting for 70 % )
从已有的5 ’部分序列到该过敏蛋白编码区为543bp ，编码181个氨基酸。开放阅读框588bp ，包括编码区的585bp及一个终止密码，可编码一个由195个氨基酸组成的功能蛋白。 3 ’端非编码区包括180个碱基，富含at ( 70 ) 。 |
